@article{715b5a66106d419790d6c69e80e128b8,
title = "Maximizing the Accessibility in DNA Origami Nanoantenna Plasmonic Hotspots",
abstract = "DNA nanotechnology has conquered the challenge of positioning quantum emitters in the hotspot of optical antenna structures for fluorescence enhancement. Therefore, DNA origami serves as the scaffold to arrange nanoparticles and emitters, such as fluorescent dyes. For the next challenge of optimizing the applicability of plasmonic hotspots for molecular assays, a Trident DNA origami structure that increases the accessibility of the hotspot is introduced, thereby improving the kinetics of target molecule binding. This Trident NanoAntenna with Cleared HOtSpot (NACHOS) is compared with previous DNA origami nanoantennas and improved hotspot accessibility is demonstrated without compromising fluorescence enhancement. The approach taps into the potential of Trident NACHOS for single-molecule-based plasmonic biosensing.",
keywords = "biosensing, DNA nanotechnology, nanoantenna hotspot, plasmonics, plasmonic hotspot accessibility, single-molecule detection, single-molecule fluorescence",
author = "Cindy Close and Kateryna Trofymchuk and Lennart Grabenhorst and Birka Lalkens and Viktorija Glembockyte and Philip Tinnefeld",
note = "Funding Information: The authors thank Vivien Behrendt, Dr. Benedikt Hauer, and Dr.‐Ing. Albrecht Brandenburg (Fraunhofer‐Institut f{\"u}r Physikalische Messtechnik IPM, Freiburg, Germany) for cooperation on the DNA origami and assay development and Prof. Tim Liedl/Prof. Joachim R{\"a}dler (Ludwig‐Maximilians‐Universit{\"a}t, Department f{\"u}r Physik, Munich, Germany) for providing access to their facilities, especially to the transmission electron microscope, and to Dr. Florian Selbach for preforming TEM measurements. C.C. thanks Maximilian Sacherer and Jakob Hartmann for help with experiments in the early project stages and Renukka Yaadav, as well as Martina Pfeiffer for fruitful cooperation on related projects. P.T. gratefully acknowledges financial support from the DFG (grant number TI 329/9‐2, project number 267681426, INST 86/1904‐1 FUGG, excellence cluster e‐conversion EXC 2089/1‐390776260), Sino‐German Center for Research Promotion (grant agreement C‐0008), BMBF (Grants POCEMON, 13N14336, and SIBOF, 03VP03891). B.L. acknowledges funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy—EXC‐2123 QuantumFrontiers—390837967 and “Nieders{\"a}chsisches Vorab” through “Quantum‐ and Nano‐Metrology (QUANOMET)” initiative within the project NL‐1. V.G. acknowledges the support from European Union's Horizon 2020 research and innovation program under the Marie Sk{\l}odowska‐Curie actions (grant agreement No. 840741). K.T. and V.G. acknowledge the support by Humboldt Research Fellowships from the Alexander von Humboldt Foundation. ",
year = "2022",
month = aug,
day = "23",
doi = "10.1002/admi.202200255",
language = "English",
volume = "9",
journal = "Advanced materials interfaces",
issn = "2196-7350",
publisher = "John Wiley and Sons Ltd",
number = "24",
}
